A 1978 graduate of the Culinary Institute of America and a 28-year veteran of professional kitchens, Bourdain is currently a chef-at-large, whose home base is Brasserie Les Halles, where he was executive chef for many years.

Anthony was born in New York City to Pierre (d.1988) and Gladys Bourdain, growing up in Leonia, New Jersey. Bourdain has French ancestry on his father's side; his paternal grandfather immigrated to New York from France following World War I. Bourdain was a student at Englewood School for Boys, graduating in 1973. He attended Vassar College, dropping out after two years. He graduated from the Culinary Institute of America in 1978. Currently, Bourdain is honorary Chef-at-Large of Brasserie Les Halles, where he held the title of executive chef for nearly a decade. When he is not travelling, Bourdain lives in Manhattan.

Bourdain married his high-school girlfriend, Nancy Putkoski, in the 1980s, remaining together for two decades before divorcing; Bourdain has cited the irrevocable changes that come from travelling widely as the cause of the split. He currently lives with his second wife, Ottavia Busia. Together, they have one daughter, Ariane, born on April 9, 2007; the couple wed on April 20, 2007. Ottavia has appeared in several episodes of No Reservations — notably the episode on Sardinia, her birthplace, and the Tuscany episode, in which she plays a disgruntled Italian diner.

In Kitchen Confidential, Bourdain describes how his love of food was kindled in France — when he tried his first oyster on an oyster fisherman's boat as a youth while on a family vacation. Later, while attending Vassar College, he worked in the seafood restaurants of Provincetown, Massachusetts, which sparked his decision to pursue cooking as a career. Bourdain graduated from the Culinary Institute of America in 1978, and went on to run various restaurant kitchens in New York City — including the Supper Club, One Fifth Avenue, and Sullivan's — culminating in the position of executive chef at Brasserie Les Halles beginning in 1998. Brasserie Les Halles is based in Manhattan, with additional locations in Miami and, at the time of Bourdain's tenure, Washington, D.C. and Tokyo, Japan.

Bourdain gained immediate popularity from his 2000 New York Times bestselling book Kitchen Confidential: Adventures in the Culinary Underbelly, an outgrowth of his now famous article in The New Yorker called "Don't Eat Before Reading This". The book is a witty and rambunctious exposé of the hidden and darker side of the culinary world, and is a memoir of Bourdain's professional life as well.

Bourdain subsequently wrote two more New York Times bestselling nonfiction books: A Cook's Tour (2001), an exotic account of his food and travel exploits across the world, written in conjunction with his first television series; and The Nasty Bits (2006), another collection of exotic, provocative, and humorous anecdotes and essays mainly centered on food. Bourdain's additional books include Anthony Bourdain's Les Halles Cookbook; the culinary mysteries Bone in the Throat and Gone Bamboo; a hypothetical historical investigation, Typhoid Mary: An Urban Historical; and No Reservations: Around the World on an Empty Stomach. His latest book, Medium Raw: A Bloody Valentine to the World of Food and the People Who Cook, the sequel to Kitchen Confidential, was published in 2010.

Bourdain's articles and essays have appeared many places, including in The New Yorker, The New York Times, The Times, The Los Angeles Times, The Observer, Gourmet, Maxim, Esquire (UK), Scotland on Sunday, The Face, Food Arts, Limb by Limb, BlackBook, The Independent, Best Life, the Financial Times, and Town & Country. On the Internet, Bourdain's blog for Season 3 of Top Chef was nominated for a Webby Award for best Blog – Cultural/Personal in 2008.

The acclaim surrounding Bourdain's racy memoir, Kitchen Confidential, led to an offer by the Food Network to host his own food and world-travel show, A Cook's Tour, which premiered on January 8, 2002. In July 2005, he premiered a new, somewhat similar television series, Anthony Bourdain: No Reservations, on the Travel Channel. As a further result of the immense popularity of Kitchen Confidential, the Fox sitcom Kitchen Confidential aired in 2005, in which the character "Jack Bourdain" is based loosely on the biography and persona of Anthony Bourdain.

In July 2006, Bourdain was in Beirut filming an episode of No Reservations when the Israel-Lebanon conflict broke out. Bourdain and his crew were evacuated with other American citizens on the morning of July 20 by the United States Marines. Because of the unexpected conflict only a few hours of footage were available from the first restaurant on their agenda. Bourdain's producers compiled the Beirut footage into a No Reservations episode which aired on August 21, 2006. Uncharacteristically, the episode included footage of both Bourdain and his production staff, and included not only their initial attempts to film the episode, but also their firsthand encounters with Hezbollah supporters, their days of waiting for news with other expatriates in a Beirut hotel, and their eventual escape aided by a "cleaner" (unseen in the footage), whom Bourdain dubbed "Mr. Wolf" after the character portrayed by Harvey Keitel in Pulp Fiction. The episode was nominated for an Emmy Award in 2007.

Bourdain has appeared five times as guest judge on Bravo's Top Chef reality cooking competition program: first in the November 2006 "Thanksgiving" episode of Season 2; and then again in June 2007 in the first episode of Season 3, judging the "exotic surf and turf" competition featuring ingredients including abalone, alligator, black chicken, geoduck and eel. His third appearance was also in Season 3, as an expert on air travel, judging the competitors' airplane meals. Bourdain also wrote weekly blog commentaries for many of the Season 3 episodes, filling in as a guest blogger while Top Chef judge Tom Colicchio was busy opening a new restaurant. Bourdain next appeared as a guest judge for the opening episode of Season 4, in which pairs of chefs competed head-to-head in the preparation of various classic dishes; and again in the Season 4 Restaurant Wars episode, temporarily taking the place of head judge Tom Colicchio, who was at a charity event.